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グローバルCOE講演会      案内ポスターのダウンロード

Prof. Marek Pruski (Iowa State University, USA)
New Strategies for Improving Sensitivity and Resolution in Solid-State NMR; Applications to Catalytic Nanoscale Materials, Biomolecules and Fossil Fuels

2011年3月11日(金)15:00-16:00、理学部6号館571号室にて、Prof. Marek Pruski氏の講演会を行います。

Abstract: Remarkable gains in sensitivity and resolution have been achieved in solid-state NMR spectroscopy by combining fast magic angle spinning (at ~ 40 kHz) with new multiple radiofrequency pulse sequences. The latest capabilities include 2D through-bond and through-space H{X} heteronuclear correlation protocols utilizing indirect detection and homonuclear multipulse H decoupling. These methods and theoretical calculations provided unique insights into the structure and dynamics of molecules bound to the surface of mesoporous silica nanoparticles. In particular, they served as a predictive tool in the design of an excellent catalyst for the esterification reaction and revealed the arrangement of surfactants inside the supramolecular-templated mesoporous materials. The new capabilities of solid-state NMR spectroscopy will also be demonstrated on a naturally abundant tripeptide (N-formyl-L-methionyl-L-leucyl-L-phenylalanine, f-MLF-OH) and a series of coals.


案内ポスターのダウンロード

グローバルCOE講演会      案内ポスターのダウンロード

Prof. Dr. Konstantin I. Momot (Discipline of Physics, Queensland University of Technology, Brisbane, Australia)
The use of spin relaxation and molecular diffusion for magnetic resonance imaging of supramolecular organisation

2010年7月13日(火)15:00-、理学部6号館571号室にて、Dr. Konstantin I. Momot氏の講演会を行います。

Abstract: In this talk we will discuss MR imaging of molecular order in anisotropic environments using the example of articular cartilage (AC). Articular cartilage is a connective tissue covering the articulating surfaces of long bones. Its structural basis is a biopolymeric extracellular matrix (ECM) based on collagen and proteoglycans. Diffusion-Tensor Imaging (DTI) enables direct measurement of the predominant alignment of collagen in AC,1,2 as well as reorganisation of collagen fibres under mechanical load.3 An alternative and potentially faster way of measuring the collagen alignment is the anisotropy of the water 1H transverse relaxation rate (R2).4 MRI R2 maps can be acquired faster than accurate diffusion-tensor maps; we will discuss how R2 anisotropy could be used as a basis of rapid mapping of collagen alignment.


案内ポスターのダウンロード

京都大学グローバルCOEプログラム 
物質科学の新基盤構築と次世代育成国際拠点
--- 統合された物質科学 ( Integrated Materials Science ) ---

日仏固体NMR雀の学校(仮称)

無事?終了しました!

記念撮影


講義の模様


実習の模様



何をするの?

・固体NMRに基本的なことについてきっちりと講義します。日本人だけでは講師陣がマンネリなのでフランス人を同人数お願いする予定です。
・講義は日本人は日本語で、フランス人はフランスなまりの英語になるでしょう。
・残り半〜1日で実際にNMRを使って実習します。希望のメーカーマシンに分かれて実習することを計画しています。
・対象は京大の学生さんと、企業に入って上司に固体NMRやれといわれて困っている人です。

日程・場所

・2010年5月12〜14日
・京都大学大学院理学研究科セミナーハウスにて。





案内ポスターのダウンロード

暫定プログラム


グローバルCOE講演会      案内ポスターのダウンロード

Prof. Dr. Dieter Suter (Technische Univasitat Dortmund, Germany)
Spins as qubits: Spin-based quantum computing

2009年12月15日(火)、理学部6号館872号室にて、NMRのエキスパートであるDr. D. SuterにNMRを用いた量子コンピュータについての講演をしてもらいます。奮ってご参加ください。

Abstract: Processing of digital information has progressed at an enormous speed over the last decades and thus become an indispensable resource. Still, for some computational problems, no efficient algorithms are known for today's computers. If quantum mechanical systems are used, instead of classical ones, some of these problems become solvable with an exponential speedup over classical computers. We will discuss some demonstration experiments, where we use magnetic resonance techniques to process quantum information stored in nuclear and electronic spins. While the computational power of today's quantum computer is rather limited, we will also discuss physical systems with the potential to become scalable quantum information processors.

グローバルCOE講演会      案内ポスターのダウンロード

Dr. Matthias Ernst (ETH Zurich, Switzerland)
Lectures on Advanced Solid-State NMR

2009年8月31日(月)-9月1日(火)、理学部6号館571号室にて、固体NMRのエキスパートであるDr. Matthias Ernstによる講演会を行います。講演は5部構成で、基礎も踏まえながら最先端の固体NMRのトピックを講義してもらいます。

案内ポスターのダウンロード

----- プログラム -----

8/31 14:00-
Talk #1 Floquet Theory in Solid-State NMR: Introduction and Examples
Talk #2 Heteronuclear Spin Decoupling Under Magic-Angle Spinning
Talk #3 Spin Diffusion in MAS Solid-State NMR

9/1 10:00-12:00
Talk #4 Low RF Power and Fast Magic-Angle Spinning: Challenges and New Opportunities
Talk #5 Echo-Phenomena in Solids: Coherent Evolution and Spin Thermodynamics


----- Abstracts -----


Talk #1 Floquet Theory in Solid-State NMR: Introduction and Examples


Abstract: The theoretical description of many experiments in solid-state NMR requires the treatment of multiple time dependencies in the Hamiltonian. The time dependencies originate from either macroscopic sample rotation (MAS, DOR, DAS) or from interaction-frame transformations if radio-frequency irradiations are involved. Treatment of time-dependent Hamiltonians is often done using Average Hamiltonian theory (AHT) [1] which is limited to a single cycle time. Treating Hamiltonians with multiple time dependencies using AHT requires approximations like a separation of time frames or the synchronization of frequencies.
This talk will give an introduction to Floquet theory [2] which will be illustrated using examples from solid-state NMR. Floquet theory has the advantage that it can describe multiple time dependencies in the Hamiltonian without making assumptions about the involved time scales of the characteristic frequencies. The emphasis will be on operator-based perturbation treatments where effective Hamiltonians can be derived in operator form [3-5] for problems of different dimensionality.

References:
[1] Ulrich Haeberlen, "High Resolution NMR in Solids - Selective Averaging", Academic Press, New York (1976).
[2] J. H. Shirley, "Solution of the Schrodinger Equation with a Hamiltonian periodic in Time" Physical Review 138B, 979 (1965).
[3] Matthias Ernst, Ago Samoson, and Beat H. Meier, "Decoupling and Recoupling Using Continuous-Wave Irradiation in Magic-Angle-Spinning Solid-State NMR: A Unified Description Using Bimodal Floquet Theory.", The Journal of Chemical Physics 123, 064102-1-10 (2005).
[4] Matthias Ernst, Helen Geen, and Beat H. Meier, "Amplitude-Modulated Decoupling in Rotating Solids: A Bimodal Floquet Approach.", Solid-State Nuclear Magnetic Resonance 29, 2-21 (2006).
[5] Ingo Scholz, Paul Hodgkinson, Beat H. Meier, and Matthias Ernst, "Understanding two-pulse phase-modulated decoupling in solid-state NMR.", The Journal of Chemical Physics, 130, 114510 (2009).



Talk #2 Heteronuclear Spin Decoupling Under Magic-Angle Spinning


Abstract: Heteronuclear spin decoupling is an essential ingredient to obtain high-resolution spectra in solids under magic-angle-spinning (MAS) conditions. [1] Initially high-power continuous-wave (cw) decoupling was used but it was observed that with increasing MAS frequencies the residual line width increases significantly. Therefore, multiple-pulse decoupling sequences have become very important at higher MAS frequencies. Analyzing the efficiency of any decoupling method in solid-state NMR under MAS requires the analysis of three different possible contributions to the line width: The residual second-order coupling terms which are typically cross terms between the heteronuclear coupling and the CSA tensor or a homonuclear coupling. For an ideal decoupling sequence these terms would be zero but this is not always possible due to the fact that there are multiple terms that can not be zeroed simultaneously. Strong homonuclear couplings between the irradiated spins gives rise to spin-diffusion that leads to spin flips. These spin flips lead to a line narrowing ("self decoupling") of the residual splitting. This process is very similar to the line narrowing observed in fast chemical exchange. Resonance conditions between the rotation in real space (MAS) and the rotations in spin space (rf irradiation) can either lead to additional line broadening if heteronuclear couplings are recoupled or to line narrowing if homonuclear dipolar couplings are recoupled and "self decoupling" is enhanced. A full analysis of decoupling sequences is only possible in an interaction frame where the rf Hamiltonian is transformed away. In such an interaction frame, the Hamiltonian is always time dependent with at least two frequencies: the MAS frequency and the basic frequency of the pulse sequence. Depending on the details of the decoupling sequence, additional frequencies can be present in the interaction frame. Time-dependent Hamiltonians with multiple frequencies can best be described using multi-mode Floquet theory [2] where incommensurate and commensurate frequencies can be treated. Using an operator-based perturbation treatment, one can obtain time-independent effective Hamiltonians. It is then possible to analyze the effective Hamiltonian in terms of the three effects discussed above. Using such an approach, one can understand the decoupling quality as a function of the experimental parameters like pulse length, rf-field amplitude and phase angles of commonly used decoupling sequences like cw decoupling, two-pulse phase-modulated (TPPM) decoupling [3], or XiX decoupling [4].

References:
[1] Matthias Ernst, "Heteronuclear spin decoupling in solid-state NMR under magic-angle sample spinning", J. Magn. Reson. 162, 1-34 (2003).
[2] Matthias Ernst, Ago Samoson, and Beat H. Meier, "Decoupling and recoupling using continuous-wave irradiation in magic-angle-spinning solid-state NMR: A unified description using bimodal Floquet theory", J. Phys. Chem. 123, 064102 (2005).
[3] Andrew E. Bennett, Chad M. Rienstra, Michele Auger, K. V. Lakshmi, Robert G. Griffin, "Heteronuclear decoupling in rotating solids", J. Chem. Phys. 103, 6951-6958 (1995).
[4] Andreas Detken, Edme H. Hardy, Matthias Ernst, and Beat H. Meier, "Simple and efficient decoupling in magic-angle spinning solid-state NMR: the XiX scheme", Chem. Phys. Lett. 356, 298-304 (2002).



Talk #3 Spin Diffusion in MAS Solid-State NMR


Abstract: Proton-driven spin diffusion (PDSD) under magic-angle spinning (MAS) is one of the most important techniques in solid-state NMR to obtain distance constraints in uniformly or specifically labeled biomolecules. Advantages of PDSD over other pulse sequences are ease of implementation, low rf-field requirements, and reduced sensitivity to dipolar-truncation effects [1]. The latter is the reason that PDSD can also be used to obtain long-range distance constraints in uniformly labeled samples. At slower MAS frequencies, the residual line broadening by the heteronuclear dipolar couplings provides compensation for chemical-shift differences. At higher spinning frequencies, active recoupling of the heteronuclear dipolar couplings by cw irradiation of the protons at the n=1 rotary-resonance condition (DARR experiment [2],[3]) is used to broaden the lines and speed up the spin-diffusion process.
The talk will emphasis three subjects related to the proton-driven spin-diffusion experiment:
(i) Dipolar truncation describes the phenomenon that transfer across small dipolar couplings is difficult to observe in the presence of large dipolar couplings. The PDSD experiment is one of the few experiments that allow the measurement of long-range distance constraints. This can be understood by analyzing the driving Hamiltonian of PDSD under MAS.
(ii) We have experimentally and theoretically investigated the spin-diffusion process under cw irradiation of the protons in more detail. Our experiments show that under cw irradiation there are distinct resonance conditions. These resonance conditions can be explained using triple-mode Floquet theory [4] as second-order resonance conditions between the homonuclear and a heteronuclear dipolar coupling. These resonance conditions explain why DARR is only effective in an intermediate spinning regime and not at fast MAS [5].
(iii) The spinning-speed dependence of PDSD is strongly dependent on the difference of the isotropic chemical shifts. Experimentally, we have observed differences in the rate constants which span a range of 5-6 orders of magnitude. Such differences in the spin-diffusion rate constant as a function of the chemical-shift difference cannot be explained by assuming Lorentzian or Gaussian line shapes for the zero-quantum line that provides the energy compensation for the spin-diffusion process. Using simple models for small spin systems, the origin of these strong dependence on the chemical-shift difference can be understood.

References:
[1] Andreas Grommek, Beat H. Meier, and Matthias Ernst, "Distance information from proton-driven spin diffusion under MAS", Chem. Phys. Lett. 427, 404-409 (2006).
[2] K. Takegoshi, Shinji Nakamura, and Takehiko Terao, "13C-1H dipolar-assisted rotational resonance in magic-angle spinning NMR", Chem. Phys. Lett. 344, 631-637 (2001).
[3] K. Takegoshi, Shinji Nakamura, and Takehiko Terao, "13C-1H dipolar-driven 13C-13C recoupling without 13C rf irradiation in nuclear magnetic resonance of rotating solids", J. Chem. Phys. 118, 2325-2341 (2003).
[4] Ingo Scholz, Beat H. Meier, and Matthias Ernst, "Operator-Based Triple-Mode Floquet Theory in Solid-State NMR", J. Chem. Phys. 127 (2007).
[5] Ingo Scholz, Theofanis Manolikas, Matthias Huber, Beat H. Meier, and Matthias Ernst, "MIRROR Recoupling And Its Application To Spin Diffusion Under Fast Magic-Angle Spinning", Chem. Phys. Lett. 460, 278-283 (2008).



Talk #4 Low RF Power and Fast Magic-Angle Spinning: Challenges and New Opportunities


Abstract: Over the past years, magic-angle spinning (MAS) frequencies have been increasing up to 70 kHz that can be achieved with commercially available 1.3 mm MAS probes. Fast MAS is always connected to rotors of smaller diameter and, therefore, also smaller sample volume. The decreased sensitivity due to the reduced sample volume is only partially compensated by a larger filling factor (thinner rotor walls). There are several reasons why experiments under faster MAS are of interest. Firstly, the resolution of NMR spectra increases since rotational-resonance conditions can be avoided even at high static fields and decoupling performance increases with increasing MAS frequencies. Secondly, a new class of NMR experiments becomes accessible where rf-field amplitudes are lower than the MAS spinning frequencies [1-4]. Such low-power experiments reduce the sample heating induced by the electric field which is especially important for heat-sensitive samples. Thirdly, the implementation of adiabatic experiments that rely on resonance conditions involving the spinning frequency become more efficient.
In this talk I will discuss the advantages and disadvantages of low-power implementations of the various polarization-transfer and free-evolution time periods in typical NMR experiments, e.g., cross polarization, homonculear polarization transfer, and decoupling during direct or indirect free evolution time.

References:
[1] Matthias Ernst, Ago Samoson, and Beat H. Meier, "Low-Power Decoupling in Fast Magic-Angle Spinning NMR", Chemical Physics Letters 348, 293-302 (2001).
[2] Matthias Ernst, Andreas Detken, Anja Bockmann, and Beat H. Meier, "NMR Spectra of a Micro-Crystalline Protein at 30 kHz MAS.", Journal of the American Chemical Society 125, 15807-15810 (2003).
[3] Matthias Ernst, Marcel A. Meier, Ago Samoson, and Beat H. Meier, "Low-Power High-Resolution Solid-State NMR of Peptides and Proteins.", Journal of the American Chemical Society 126, 4764-4765 (2004).
[4] Adam Lange, Ingo Scholz, Theofanis Manolikas, Matthias Ernst, and Beat H. Meier, "Low-power cross polarization in fast magic-angle spinning NMR experiments.", Chemical Physics Letters 468, 100-105 (2009).



Talk #5 Echo-Phenomena in Solids: Coherent Evolution and Spin Thermodynamics


Abstract: Since the first demonstration of spin echoes in 1950 by Erwin Hahn [1], they have attracted a lot of attention since they demonstrate the coherent time evolution of spin systems even if they appear to decay. This duality can be represented in the fact that on one hand we can describe many experiments in strongly-dipolar coupled spin systems using thermodynamic concepts where echo phenomena do not exist. But on the other hand, we can also describe them using the coherent time evolution of the spin system where we can induce echoes by inverting the sign of the Hamiltonian. The concepts and the limitations of the descriptions will be discussed based on spin-echo experiments in spin diffusion and in cross polarization under MAS.

References:
[1] Erwin L. Hahn, "Spin Echoes", Physical Review 80, 580 (1950).
[2] Susan M. De Paul, Marco Tomaselli, Alexander Pines, Matthias Ernst, and Beat H. Meier, "Reversal of Radio-Frequency Driven Spin Diffusion by Reorientation of the Sample Spinning Axis", The Journal of Chemical Physics 108, 826-829 (1998).
[3] Matthias Ernst, Beat H. Meier, Marco Tomaselli, and Alexander Pines, "Time Reversal of Cross Polarization in Solid-State NMR", Molecular Physics 95, 849-858 (1998).




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案内ポスターのダウンロード



グローバルCOE講演会     

2009年5月12日(火)13:30より、理学部6号館571号室にて、固体NMRのエキスパートである

  • Prof. Jean-Paul Amoureux
  • Dr. Piotr Tekely
の2氏による講演会を行います。

----- プログラム -----

13:30-14:30
Prof. Jean-Paul Amoureux    (University of Lille, France)

Optimized recoupling & decoupling schemes for ultra-fast MAS and high-field spectrometers


Abstract:I will show the way methodological groups, such like mine, try to adapt to solid-state NMR the methods that are presently used routinely in solution state. I will also show on several examples how we try to overcome the limitations of the previously proposed methods for solid-state. In this purpose I will present some of the decoupling and recoupling methods we have developed last year and that are optimized for high-field spectrometers (> 14 T) and ultra-fast MAS (ν > 30 kHz). These methods are of two types: high-resolution of protons, and homo-nuclear correlations of spin-1/2 or quadrupolar nuclei. I will also present: (i) one method that allows to do H/C/N structural analyses of biological compounds without any enrichment, and (ii) how to use the covariance treatment in homo-nuclear methods, in order to decrease the experimental time by a factor 〜10.


13:30-14:30
Dr. Piotr Tekely    (Research director in CNRS, Ecole Normale Superieure, France)

Probing molecular geometry and intermolecular contacts by solid-state NMR. Methodological aspects and applications


Abstract: In this talk we shall deal with some methods and approaches developed for investigating molecular geometry and intermolecular contacts in systems of biological interest. Appropriate description and understanding of the nature of intermolecular contacts in hydrogen-bonded systems remains an important and challenging problem. In the first part we will present results of a solid-state NMR study of a series of enantiomers and racemates of o-phosphorylated amino acids aiming to probe the dependence of magnetic shielding on the nature and the strength of hydrogen bonding contacts. An attractive possibility of gaining deeper insight into the ionisation state of different functional group during successive steps of deprotonation will be also discussed. We will pay special attention to the studies of the ionisation state through the use of chemical shift tensor fingerprints. In the second part of more methodological character, new solid-state NMR methods will be presented for exploiting dipolar interactions in structural studies with improved sensitivity and resolution.


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