Welcome to the website for FUJIHASHI MASAHIRO! 

updated on July 12/2008 

Profile 

Masahiro Fujihashi, Ph. D.

        Date of Birth :      

Place of Birth :

September, 1973

 Kobe, JAPAN

Assistant Professor
Department of Chemistry, Graduate School of Science, Kyoto University
Kitashirakawa-oiwake, Sakyo, Kyoto, 606-8502, JAPAN

Tel:    +81-75-753-4030
Fax:    +81-75-753-4032

Email:
   account = mfuji
   Domain = kuchem.kyoto-u.ac.jp
Please reconstruct my email address like account@domain.

 

Carrier 

October 2004 - Assistant Professor / Instructor
Biological Structural Chemistry,  Department of Chemistry, 
Graduate School of Science,  Kyoto University
April 2004 -
 September 2004  
JSPS  Research Fellow
Laboratory of Supramolecular Crystallography,
Institute for Protein Research, Osaka University, Japan
Working in
  Pai Laboratory,  Ontario Cancer Institute,
  University Health Network, Canada
  to continue the research from 2002
 
March 2002 - 
   March 2004    
Postdoctoral Fellow
Pai Laboratory,  Ontario Cancer Institute,
University Health Network, Canada
  JSPS  Overseas Research Fellow (2002-2004)

April 2001 -
   March 2002     
Postdoctoral Fellow
Miki Laboratory,  Department of Chemistry, 
Graduate School of Science,  Kyoto University
 

Education 

March, 2001    Ph.D
Dissertation: Structural study on cis-type prenyl chain elongating enzyme.
1996 - 2001 Miki Laboratory,  Department of Chemistry, 
Graduate School of Science,  Kyoto University  
1992 - 1996 Faculty of Science,   Kyoto University  

Publication 

1: "Crystal structure of human cyclophilin D in complex with its inhibitor, cyclosporin A at 0.96-A resolution.";
Kajitani, K., Fujihashi, M., Kobayashi, Y., Shimizu, S., Tsujimoto, Y. & Miki, K. (2008). Proteins 70, 1653-9

2: "A potent, covalent inhibitor of orotidine 5'-monophosphate decarboxylase with antimalarial activity.";
Bello, A.M., Poduch, E., Fujihashi, M., Amani, M., Li, Y., Crandall, I., Hui, R., Lee, P.I., Kain, K.C., Pai, E.F.& Kotra, L.P. (2007). J Med Chem. 50, 915-21

3: "Crystal structure of archaeal photolyase from Sulfolobus tokodaii with two FAD molecules: implication of a novel light-harvesting cofactor."
Fujihashi, M., Numoto N, Kobayashi Y, Mizushima A, Tsujimura M, Nakamura A, Kawarabayasi Y, Miki K. (2007) J Mol Biol. 365, 903-10.

4. "The crystal structure of (S)-3-O-geranylgeranylglyceryl phosphate synthase reveals an ancient fold for an ancient enzyme";
Payandeh, J., Fujihashi, M., Gillon, W.& Pai, E.F. (2006). J Biol Chem 281, 6070-8.

5. "Design of Inhibitors of Orotidine Monophosphate Decarboxylase Using Bioisosteric Replacement and Determination of Inhibition Kinetics";
Poduch, E., Bello, A.M., Tang, S., Fujihashi, M., Pai, E.F.& Kotra, L.P. (2006). J Med Chem 49, 4937-4945.

6. "An unprecedented twist to ODCase catalytic activity";
Fujihashi, M., Bello, A.M., Poduch, E., Wei, L., Annedi, S.C., Pai, E.F.& Kotra, L.P. (2005). J Am Chem Soc 127, 15048-50.

7. "Involvement of tyrosines at fucose-binding sites of Aleuria aurantia lectin: non-equal response to site-directed mutagenesis among five sites";
Amano, K., Fujihashi, M., Ando, A., Miki, K.& Nagata, Y. (2004). Biosci Biotechnol Biochem 68, 841-7.

8. "The crystal structure of an oxidatively stable subtilisin-like alkaline serine protease, KP-43, with a C-terminal beta-barrel domain";
Nonaka, T., Fujihashi, M., Kita, A., Saeki, K., Ito, S., Horikoshi, K.& Miki, K. (2004). J Biol Chem 279, 47344-51.

9. "Crystal structure of fucose-specific lectin from Aleuria aurantia binding ligands at three of its five sugar recognition sites";
Fujihashi, M., Peapus, D.H., Kamiya, N., Nagata, Y.& Miki, K. (2003). Biochemistry 42, 11093-9. Selected as a hot paper from the Journal!

10. "X-ray crystallographic characterization and phasing of a fucose-specific lectin from Aleuria aurantia";
Fujihashi, M., Peapus, D.H., Nakajima, E., Yamada, T., Saito, J.I., Kita, A., Higuchi, Y., Sugawara, Y., Ando, A., Kamiya, N., Nagata, Y.& Miki, K. (2003). Acta Crystallogr D Biol Crystallogr 59, 378-80.

11. "Mutational analysis of allylic substrate binding site of Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase";
Fujikura, K., Zhang, Y.W., Fujihashi, M., Miki, K.& Koyama, T. (2003). Biochemistry 42, 4035-41.

12. "Significance of highly conserved aromatic residues in Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase";
Kharel, Y., Zhang, Y.W., Fujihashi, M., Miki, K.& Koyama, T. (2003). J Biochem (Tokyo) 134, 819-26.

13. "Crystal structure of calcium-free alpha-amylase from Bacillus sp. strain KSM-K38 (AmyK38) and its sodium ion binding sites";
Nonaka, T., Fujihashi, M., Kita, A., Hagihara, H., Ozaki, K., Ito, S.& Miki, K. (2003). J Biol Chem 278, 24818-24.

14. "Crystal structure of cis-prenyl chain elongating enzyme, undecaprenyl diphosphate synthase";
Fujihashi, M., Zhang, Y.W., Higuchi, Y., Li, X.Y., Koyama, T.& Miki, K. (2001). Proc Natl Acad Sci U S A 98, 4337-42.

15. "Identification of Significant residues for homoallylic substrate binding of Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase";
Kharel, Y., Zhang, Y.W., Fujihashi, M., Miki, K.& Koyama, T. (2001). J Biol Chem 276, 28459-64.

16. "Crystallization and preliminary X-ray diffraction studies of a novel alkaline serine protease (KP-43) from alkaliphilic Bacillus sp. strain KSM-KP43";
Nonaka, T., Fujihashi, M., Kita, A., Saeki, K., Ito, S.& Miki, K. (2001). Acta Crystallogr D Biol Crystallogr 57, 717-8.

17. "Crystallization and preliminary X-ray diffraction studies of undecaprenyl diphosphate synthase from Micrococcus luteus B-P 26";
Fujihashi, M., Shimizu, N., Zhang, Y.W., Koyama, T.& Miki, K. (1999). Acta Crystallogr D Biol Crystallogr 55, 1606-7.

 

Awards

2004 JSPS (Japan Society for Promotion of Science) Research Fellow
2004 Uehara Memorial Foundation Reserch Fellowship
(declined due to JSPS Research Fellowship)
2004 Travel Award to attend PRICPS (1st Pacific-Rim International Conference
on Protein Science) from PRICPS
2002-2004    JSPS Overseas Reserch Fellow
2002 Uehara Memorial Foundation Postdoctoral Fellowship
(declined due to receive JSPS overseas Fellowship)
2000 Travel Award to attend AsCA (Asian Crystallographic Association) from Kyoto University


 

 

 

 

 

 

Other Publication

JISTEC News (2004) Vol. 51,  P.15-16 (Japanese)