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Protocols

Basic Data
  • List of Good's Buffers
  • Codon Table
  • Size markers of DNA
  • Semi-log graph paper (Download the png file to print semi-log graph paper)
  • Multiple cloning sites of pUC and pBluescript vectors
  • Ambiguity codes
  • DNA
  • Linear polyacrylamide carrier useful in the ethanol precipitation of nucleic acids

  • Cloning of PCR products (one-tube reaction)
  • Cloning of PCR products (standard procedure)
  • Transformation of E. coli
  • Transformation of E. coli (calcium chloride method)
  • Color selection of E. coli harboring recombinant plasmid
  • Colony cracking: quick test for inserts in plasmids
  • PCR from bacterial colonies

  • Purification of Plasmid (Miniprep)
  • Purification of Plasmid (Midiprep)

  • Recovery of DNA from low melting point (LMP) agarose
  • Small scale preparation of plasmid with FlexiPrep Kit (Pharmacia)
  • Recovery of DNA from agarose gel with BandPrep Kit (Pharmacia)

  • DNA sequencing with the BcaBest Dideoxy Sequencing Kit (TAKARA)
  • Random labeling of DNA with BcaBest DNA polymerase
  • Southern hybridization
  • PROTEIN
  • Ammonium sulfate precipitation of proteins
  • SDS-polyacrylamide gel electrophoresis
  • Semi-dry electrophoretic transfer of protein onto nitrocellulose membrane
  • Immunological detection of proteins on membrane (western blotting)
  • Affinity of selected classes of monoclonal antibodies for protein A
  • RNA
  • Purification of RNA from plant tissues
  • Small-scale purification of RNA from plant tissues
  • Preparation of poly(A)+ RNA with Oligotex-dT30
  • Northern hybridization
  • ARABIDOPSIS
  • Medium for Arabidopsis thaliana
  • Sterilization of seeds
  • OTHER SITES
  • Biowww.net
  • Nucleic Acids Research Methods
  • Protocol Online
  • Biotechnical Forum (in Japanese)
  • Laboratory protocols (protocols for ciliates)