Basic Data

List of Good's Buffers

Codon Table

Size markers of DNA

Semi-log graph paper (Download the png file to print semi-log graph paper)

Multiple cloning sites of pUC and pBluescript vectors

Ambiguity codes

DNA

Linear polyacrylamide carrier useful in the ethanol precipitation of nucleic acids

Cloning of PCR products (one-tube reaction)

Cloning of PCR products (standard procedure)

Transformation of E. coli

Transformation of E. coli (calcium chloride method)

Color selection of E. coli harboring recombinant plasmid

Colony cracking: quick test for inserts in plasmids

PCR from bacterial colonies

Purification of Plasmid (Miniprep)

Purification of Plasmid (Midiprep)

Recovery of DNA from low melting point (LMP) agarose

Small scale preparation of plasmid with FlexiPrep Kit (Pharmacia)

Recovery of DNA from agarose gel with BandPrep Kit (Pharmacia)

DNA sequencing with the BcaBest Dideoxy Sequencing Kit (TAKARA)

Random labeling of DNA with BcaBest DNA polymerase

Southern hybridization

PROTEIN

Ammonium sulfate precipitation of proteins

SDS-polyacrylamide gel electrophoresis

Semi-dry electrophoretic transfer of protein onto nitrocellulose membrane

Immunological detection of proteins on membrane (western blotting)

Affinity of selected classes of monoclonal antibodies for protein A

RNA

Purification of RNA from plant tissues

Small-scale purification of RNA from plant tissues

Preparation of poly(A)+ RNA with Oligotex-dT30

Northern hybridization

ARABIDOPSIS

Medium for Arabidopsis thaliana

Sterilization of seeds

OTHER SITES

Biowww.net

Nucleic Acids Research Methods

Protocol Online

Biotechnical Forum (in Japanese)

Laboratory protocols (protocols for ciliates)