List of Good's Buffers


Good's Buffers are a group of non toxic buffers described by Good et al. that have limited effect on biochemical reactions and very low absorbance between 240 nm and 700 nm. They do not interfere with most biochemical reactions and thus suitable for biochemical studies. (Reference: Good, N.E., Winget, G.D., Winter, W., Connolly, T.N., Izawa, S. and Singh, R.M.M., Hydrogen ion buffers for biological research. Biochemistry, 5, 467-477.)

Name pKa at 20°C ΔpKa/°C Solubility at 0°C (mol/l)
MES 6.15 -0.011 0.65
ADA 6.62 -0.011 0.09
PIPES 6.80 -0.009 1.4
ACES 6.88 -0.020 0.22
BES 7.15 -0.016 3.2
MOPS 7.20 -0.011 3.1
TES 7.50 -0.020 2.6
HEPES 7.55 -0.015 2.3
HEPPS 8.00 -0.011 1.6
TRICINE 8.15 -0.021 0.8
BICINE 8.35 -0.018 1.1
CHES 9.55 -0.011 1.15





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