Ammonium sulfate precipitation of proteins

Proteins are precipitated from aquaous solutions by dissolving ammonium sulfate into the protein solution. The following table shows the weight (g) of ammonium sulfate to be added to one litre of solution to produce a desired change in the concentration (% saturation) of ammonium sulfate. Precipitation of proteins is conventionally carried out at 0°C to avoid possible denaturation of proteins, although the % saturation values shown in the table are based on the values at 25°C described by Green and Hughes in Methods Enzymol. 1, 67-90 (1955).

%10 15 2025303335404550556065707580859095100
05684114144176196209243277313351390430472516561610662713767
10 285786118137150183216251288326365406449494540592640694
15 285788107120153185220256294333373415459506556605657
20 29597891123155189225262300340382424471520569619
25 30496193125158193230267307348390436485533583
30 19306294127162198235273314356401449496546
33 124374107142177214252292333378426472522
35 316394129164200238278319364411457506
40 316397132168205245285328375420469
45 326599134171210250293339383431
50 3366101137176214256302345392
55 3367103141179220264307353
60 3469105143183227269314
65 3470107147190232275
70 3572110153194237
75 3674115155198
80 3877117157
85 3977118
90 3877
95 39





To index

To Shiraishi's Home Page
To Biochmistry Lab. Home Page