Affinity of selected classes of monoclonal antibodies for protein A

Antibody Affinity Binding pH Elution pH
Human IgG1 very high 6.0-7.0 3.5-4.5
Human IgG2 very high 6.0-7.0 3.5-4.5
Human IgG3 low-none 8.0-9.0 lower than 7.0
Human IgG4 low-high 7.0-8.0 3.0-6.0
Mouse IgG1 low 8.0-9.0 4.5-6.0
Mouse IgG2a moderate 7.0-8.0 3.5-5.5
Mouse IgG2b high 7.0 3.0-4.0
Mouse IgG3 low-high 7.0 3.5-5.5





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