The following protocol is for TaKaRa BcaBEST Labeling Kit (cat No. 6046).

1. Mix the following solutions.

Template DNA: 5-500 ng
Random Primer: 2ul
Fill up to make a final volume of 7 ul with water.

2. Denature the template DNA for 3 min in a boiling water bath. Chill on ice.

3. Add the followings.

10x buffer: 1.25 ul
dNTP mixture: 1.25 ul
[a-32P]dCTP (111TBq/mmol, 370MBq/ml): 2.5 ul
BcaBEST DNA polymerase: 0.5 ul

4. Incubate the tube for 10 min at 50-55 C.

5. Add 0.8 ul of 0.5M EDTA.

6. Denature the DNA for 3 min in a boiling water bath. Chill on ice.

7. Add an appropriate amount of the solution into a hybridization solution.

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