研究内容・業績

研究テーマ

　　蛍光寿命画像顕微鏡を用いた葉緑体の光応答観察

Chloroplasts in plants and algae show autofluorescence of chlorophyll a mainly from photosystem II, its light-harvesting complexes, and to a less extent from photosystem I and its antenna. The lifetime of fluorescence reflects photochemical reaction in chloroplasts. We have applied fluorescence lifetime imaging microscopy (FLIM) to chloroplasts in a green algae, in order to study fine structures of chloroplast in situ and their changes. Three dimensional structures inside the chloroplasts and the dependence of the fluorescence lifetime on excitation laser intensity are studied.


研究業績

1. 蛍光寿命画像顕微法を用いたホシツリモ葉緑体の観察
　　「第20回 光合成の色素系と反応中心に関するセミナー」, P-8, 大阪, 2012年6月

2. 蛍光寿命画像顕微鏡による葉緑体微細構造の観察
「第51回日本生物物理学会年会」,3P-254, 京都, 2013年10月