Kyoto University DNA Nanotechnology Group

DNA nanotechnology

In the DNA nanotechnology group, we are creating DNA molecular nanodevices and functional materials,
and developing single-molecule imaging and analysis system for understanding biological phenomena.

Research

All existing things in nature, for example, cells in animals and plants are built up by assembly and organization of the predefined molecules, and their functions are expressed by the reaction of the molecules in a highly ordered fashion. We are learning the nature’s system and mimicking it to create a new system in a small scale. So if we artificially design nanoscale structures and precisely incorporate molecules there, it could be an ultimate technology not only for studying basic science but also for creation of new materials and devices for physical and biological applications. Using a DNA molecule as a basic component, we are working on developing novel technologies applicable for imaging, sensing, creation of nanosystem and nanodevice, and delivery system by combining designable DNA nanoscale structure & space (bioarchitecture) and chemistry.

DNA is one of the most promising molecules for preparing various nanoscale structures and spaces. This allows us to integrate multiple functional molecules and nanomaterials onto a designed nanostructure and also to produce a large-scale complicated architecture. Using an innovative DNA self-assembly system “DNA origami”, our group focuses on the following research topics. (1) Design and construction of the novel 2D and 3D DNA nanostructures and spaces; (2) Programmed assembly of nanostructures into the 2D and 3D architectures; (3) Regulation of chemical and enzymatic reactions in a designed nanospace; (4) Visualization and analysis of biomolecules in a designed nanostructure; (5) Analysis of physical properties of biomolecules in a designed nanospace; (6) Development of novel DNA crystals; (7) Development of molecular nanodevices; (8) Applications for photonic nanomaterials; (9) Applications for molecular machines and robots; (10) Development of novel delivery system for cellular applications; (11) Diagnosis and medical applications. We are using the high-speed AFM imaging system for visualizing the dynamics of molecules and nanostructures.

NEWS新着情報

3/19/2021: We joined the 101st Annual Meeting of the Chemical Society Japan (on-line). (3/19-22)
3/13/2021: Collaborative research with Prof. Yoshiki Nishimura (Kyoto University) on DNA binding protein CreHDB1 in chloroplast was accepted to PNAS.
10/15/2020: The research of our collaborator Dr. Ioanna Mela (Univ. Cambridge) was featured in the European branch of CGTN as a short documentary.
https://www.youtube.com/watch?v=ndrUFSCDyDw
10/1/2020: Dr. Yihong Feng moved to National Institute for Basic Biology.
9/23/2020: Ms. Yihong Feng obtained Ph. D degree (Graduate School of Science, Kyoto University).
4/1/2020: New undergrad and graduate students joined our group.
3/18/2020: Our research reseults were introduced in JSPS research topics.
12/3/2019: Endo visted College of Chemistry and Molecular Sciences, Wuhan University (Prof. Xiang Zhou) and gave a talk.
12/2/2019: Endo visted College of Life Science and Technology, Jinan University (Dr. Xiwen Xing) and gave a talk.
Endo visted School of Chemistry, Sun Yat-sen University (Prof. Liang Xu) and gave a talk.
11/22/2019: Prof. Bodin Tuesuwan (Chulalongkorn University) joined our group.
11/5/2019: Dr. Ioanna Mela (University of Cambridge) visted our graoup.
10/29/2019: We joined the 46th International Symposium on Nucleic Acid Chemistry (ISNAC2019), Tokyo (10/29-31).
9/26/2019: We joined The 57th Annual Meeting of the Biophysical Society of Japan, Miyazaki (9/24-26).
9/18/2019 　　　　　　　: The collaborative research with Prof. Masahiro Takinoue was press released from Tokyo Institute of Technology.
9/15/2019: We joined Kyoto University Academic Day 2019.
8/28/2019: Dr. Xiwen Xing (Jinan Univ.) visited our group. (June-August)
7/22/2019: We joined Commemorative International Symposium of the Japan Society of Nucleic Acids Chemistry 2019 (CISNAC) (Konan Univ. Kobe). (7/22-24)
7/3/2019: Endo stayed Hanbin Mao Lab and visited Thorsten Schmidt Lab in Kent Stat University (Kent, OH, USA). (7/3-19) 　　　　　　　
6/28/2019: Endo talked in symposium on molecular motor (National Genetic Research, Mishima). (6/27-28)
6/25/2019: Endo talked in 10th International Conference on Materials for Advanced Technologies (ICMAT2019) (Marina Bay Sands, Singapore). (6/23-26)
6/13/2019: Endo talked in Young Scientist Salon (Kyoto University).
5/28/2019: Endo talked in NANTECH2019 (Aalto University, Helsinki, Finland). (5/26-29)
4/15/2019: We joined FNANO19(Snowbird, UT, USA). (4/15-18)
3/19/2019: We joined the 99th Annual Meeting of the Chemical Society Japan (Konan University). (3/16-19)
3/14/2019: We joined Molecular Robotics annual meeting (Tokyo Institute of Technology).（3/14-15）
12/3/2018: Prof. Bodin Tuesuwan (Chulalongkorn University) joined our group.
11/12/2018: Dr. Sébastien Lyonnais (Centre d'étude des maladies infectieuses et pharmacologie anti-infectieuse CEMIPAI, France) joined our group.
11/7/2018: We held the 45th International Symposium on Nucleic Acid Chemistry (ISNAC), Kyoto University. (11/7-9)
10/3/2018: Mr. Shubham Mishra (Indian Institute of Science) joined our group.
9/7/2018: We joined the 12th Symposium on Biorelevant Chemistry (Osaka University). (9/9-11)
9/6/2018: The collaborative research with Prof. Hanbin Mao (Kent State University) was press-released from Kyoto University.
8/26/2018: We attended the International Roundtable on Nucleosides, Nucleotides and Nucleic Acids (IRT2018) (UC San Diego). (8/26-30)
8/9/2018 　　　　　　　: High school students visited our lab and did DNA origami experiment.
8/1/2018 　　　　　　　: The collaborative research with Prof. Hanbin Mao (Kent State University) on physical properties of G-quadruplex and i-motif in a confined space was accepted to PNAS. 　　　　　　　
7/24/2018 　　　　　　　: The collaborative research with Prof. Hisashi Tadakuma was press released from Osaka University. 　　　　　　　
6/25/2018: We joined the 10th iCeMS retreat (Hikone). (6/25-26)
6/15/2018: Collaborative research with Prof. Hisashi Tadakuma (Osaka University) on Gene Logic Chip was accepted to Nature Nanotechnology.
4/2/2018: New undergrad and graduate students joined our group.
3/20/2018: We joined the 98th Annual Meeting of the Chemical Society Japan (Nihon University). (3/20-23)
3/5/2018: We joined Molecular Robotics annual meeting (Tohoku University). (3/5-6)
3/4/2018: Endo talked in Shizuoka Life Science Symposium (Shizuoka University).
2/27/2018: Endo talked in SPIRITS symposium (kyoto University).
1/15/2018: Prof. Hiroshi Sugiyama won the CSJ Awards 2017.
1/9/2018: Prof. Bodin Tuesuwan (Chulalongkorn University) joined our group.
11/14/2017: We joined the 44th International Symposium on Nucleic Acid Chemistry (ISNAC), Tokyo (11/14-16).
10/27/2017: We joined the 9th iCeMS retreat (Hikone). (10/27-28)
10/3/2017: Prof. Kiat Ruxrungtham (Vice President, Chulalongkorn University) visited our group.
9/25/2017: We joined DNA23 (University of Texas, Austin). (9/25-28)
9/15/2017: The collaborative research with Prof. Hirohide Saito was press released from CiRA, Kyoto University. 　　　　　　　
9/7/2017: We joined the 11th Symposium on Biorelevant Chemistry (University of Tokyo). (9/7-9)
7/14/2017: We joined the Molecular Robotics Symposium (University of Tokyo).
6/22/2017: Ms. Caroline Rossi-Gendron (Ecole Normale Supérieure) joined our group (JSPS Summer Program).
6/12/2017: Endo visited Kent State University, Hanbin Mao Lab(Kent, OH, USA). (June 12-14).
6/5/2017: Endo joined Unconventional Computation and Natural Computation 2017 (UCNC 2017) conference (University of Arkansas. Fayetteville, AR, USA). (June 5-9).
5/15/2017: Ms. Djamouna Sihou (University of Bordeaux) joined our group.
5/15/2017: Kyoto University Press Release, tiltled "Disentangling chloroplast genetics. Kyoto University isolates a critical gene for plant health." collaborative research with Prof. Yoshiki Nishimura.
4/14/2017: Collaborative research with Prof. Yoshiki Nishimura (Kyoto University) on Holliday junction resolvase in chloroplast was accepted to Science.
4/11/2017: We joined FNANO17 (Snowbird, UT, USA). (4/11-14)
4/4/2017: We joined the 253rd American Chemical Society National Meeting (San Francisco, CA). (4/4-7) 　　　　　　
4/3/201７: New undergrad and graduate students joined our group.
4/1/201７: Dr. Yosuke Katsuda moved to Department Applied Chemistry and Biology, Kumamoto University as an assistant professor.
3/28/2017 　　　　　　　: Kyoto University Press Release, tiltled "Nanocages dramatically facilitate structure formation of biomolecules."　　
3/21/2017: Dr. Xiwen Xing joined our group.
3/16/2017: We joined the 97th Annual Meeting of the Chemical Society Japan (Keio University). (3/16-19)
3/11/2017: We joined the Molecular Robotics annual meeting (University of Tokyo). (3/11-13)
2/6/2017: Collaborative research with Prof. Hanbin Mao (Kent State University) on G-quadruplex in confined nanospaces was accepted to Nature Nanotechnology.
2/2/2017: We joined The 3rd SPIRITS international symposium (Kyoto University).
1/16/2017: We joined Chemical Biology Mini-sumposium at KIT.
11/26/2016: We joined The 54th Annual Meeting of the Biophysical Society of Japan, Tsukuba (11/26-27).
11/16/2016: Prof. Ilko Bald (Universität Potsdam) visited our group.
9/27/2016: We joined the 43rd International Symposium on Nucleic Acid Chemistry (ISNAC), Kumamoto (9/27-29).
9/13/2016: Endo visited Sturla Lab, ETH Zürich (Switzerland).
9/4/2016: We joined DNA22(Ludwig-Maximilians-Universität München, Germany).(9/4-9)
7/22/2016: We joined The 38th Annual Meeting of the Japanese Society for Photomedicine and Photobiology (Kyoto). (7/22-23)
7/13/2016: Ms. Tracy Guo (Tufts University) worked in our group (Amgen Scholar Programs).
6/29/2016: Endo visited Hanbin Mao Lab, Kent State University (Kent, OH, USA). (6/29-7/9)
6/15/2016: We joined the 11th Annual Meeting of the Japanese Society for Chemical Biology (Kyoto). (6/15-17)
5/30/2016: We joined the 8th iCeMS retreat (Hikone). (5/30-31)
5/24/2016: Prof. Shana Sturla (ETH Zürich) visited our group.
4/19/2016: Prof. Peter Beal (UC Davis) visited our group.
4/11/2016: We joined FNANO16 (Snowbird, UT, USA). (4/11-14)
4/1/2016: New undergrad and graduate students joined our group.
4/1/2016: Dr. Yuki Suzuki moved to Frontier Research Institute for Interdisciplinary Sciences, Tohoku University as an assistant professor.
4/1/2016: Dr. Seigi Yamamoto moved to Department of Biomedical Science and Engineering, The University of Tokushima as an assistant professor.
3/24/2016: We joined the 96th Annual Meeting of the Chemical Society Japan (Doshisha University). (3/24-27)
3/16/2016: We joined the 251st American Chemical Society National Meeting (San Diego, CA). (3/16-17)
3/15/2016: Endo joined and talked in Ten Years of DNA Origami symposium, California Institute of Technology (Pasadena, CA). (3/14-15)
2/20/2016: Molecular Robotics Young Researcher's meeting was held in iCeMS, Kyoto University. (organizer Yuki Suzuki) 　　　　　　　
2/10/2016: Mr. Michael Heinrich Räz (ETH Zürich) joined our group and worked for five months (Swiss-JSPS exchange program).
2/4/2016: Endo joined and talked in the 2nd SPIRITS international symposium (Kyoto University).
1/28/2016: We joined nano tech 2016 (Tokyo Big Sight). (1/28-29)
1/25/2016: We held the University of Bordeaux-Kyoto University Mini-Symposium on Biomolecular Science (Kyoto University).
1/23/2016: We held the annual meeting of Molecular Robotics (Kyoto University).
12/16/2015: Endo joined and talked in the 1st SPIRITS international symposium (Honolulu, HI). We invited Prof. Tim Liedl (Ludwig-Maximilians University) and Prof. Stephan Link (Rice University).
12/15/2015: We joined 2015 International Chemical Congress of Pacific Basin Societies (PacifiChem 2015) (Honolulu, HI). (12/15-19)
12/7/2015: Endo joined and talked in 601. Wilhelm und Else Heraeus-Seminar, DNA Nanotechnology Meets Plasmonics, Physikzentrum Bad Honnef, Germany. (12/7-11)
11/14/2015: We joined the SIG-MBI meeting 2015. Keio University, Yokohama.
11/12/2015: We joined the Eighth Annual Meeting of the Japanese Society for Cell Synthesis Research. Osaka University, Suita (11/12-13).
11/5/2015: Endo talked in the 2nd Kobe mini-symposium on functionalized organic molecules, Kobe University, Kobe.
10/27/2015: We joined the Chem-Bio Informatics Society (CBI) annual meeting 2015, Tokyo (10/27-28).
10/14/2015: We joined the Sekisu Chemical Forum, Tokyo.
10/13/2015: Ms. Elena Willner (Technische Universität München) joined to our group and carried out research for six months (interunversity exchange program). 　　　　　　　
9/28/2015: Prof. Hanbin Mao (Kent State University) visited our group and gave a lecture.
9/23/2015: We joined the 42nd International Symposium on Nucleic Acid Chemistry (ISNAC), Himeji (9/23-25).
9/15/2015: We joined the 64th Polymer meeting, Sendai (9/15-16).
9/10/2015: We joined the 9th Symposium on Biorelevant Chemistry, Kumamoto (9/10-12).
9/9/2015: Dr. Ioanna Mela (University of Cambridge) stayed and worked in our group.
Mr. Prakash Shrestha (Kent State University) stayed and worked in our group as an internship graduate student.
9/7/2015: We joined the 7th iCeMS retreat (Minamiyamashiro)(9/7-8).
8/28/2015: Dr. Yangyang Yang moved to East China University of Science and Technology as a reseach associate.
8/27/2015: Kyoto University Press Release, tiltled "Micrometer sized two-dimentional assembly of DNA origami on the lipid bilayer."　　
8/17/2015: We joined the DNA 21 (Harvard University, Cambridge, MA) (8/17-21).
7/16/2015: Endo talked in the 52nd Phermaceutics meeting (Kaga) (7/15-16).
6/4/2015: We joined kick-off symposium of SPIRITS (Instute of Chemistry, Kyoto University).
5/9/2015: We joined Molecular Robotics meeting for new researchers (Tokyo Institute of Technology).
5/4/2015: Endo talked in the 5th International Conference on Nucleic Acid-Protein Chemical and Structural Biology for Drug Discovery, Sichuan University (Chengdu, China)(5/3-4).
4/13/2015: We joined FNANO14 (Snowbird, UT, USA)(4/13-14).
3/27/2015: We joined the 95th Annual Meeting of the Chemical Society Japan (Funabashi). (3/26-29).
3/10/2015: We joined the Molecular Robotics annual meeting (Ito).
3/6/2015: Endo talked in the 8th symposium of Molecular Nanotechnology Center, University of Hyogo (Himeji).
2/5/2015: Endo was introduced in Angewandte Chemie Author Profile. Author Profile Masayuki Endo, Angewandte Chemie International Edition Vol. 54, page 2002 (2015).
11/13/2014: We joined the annual meeting 7.0 of the Japanese Society for Cell Synthesis Research (Tokyo).
11/5/2014: We joined the 41st International Symposium on Nucleic Acid Chemistry (ISNAC), Kokura (11/5-7).
10/2/2014: We joined the 6th iCeMS retreat (Miyazu).
9/24/2014: Ms. Xiwen Xing (Wuhan Univ.) stayed and worked in our group as an internship graduate student for six months.
9/23/2014: Prof. Ned Seeman (New York Univ.) visited out group.
9/22/2014: DNA20 was held at Shiran-Kaikan at Kyoto University (9/22-25).
9/11/2014: We joined the 8th Symposium on Biorelevant Chemistry, Okayama.
8/19/2014: The collaborative research with Prof. Hirohide Saito was press released from CiRA, Kyoto University.
7/22/2014: Prof. Bodin Tuesuwan (Chulalongkorn Univ.) visited our group and carried out the research.
7/5/2014: Endo gave a talk in a seminar of Department of Chemistry and Biotechnology, University of Tokyo (Tokyo).
5/26/2014: Mr. Guruprasad Raghavan (Indian Institute of Technology, Madras) stayed our group as an internship student and conducted research.
4/14/2014: We joined FNANO14 (Snowbird, UT, USA).
3/27/2014: We joined the 94th Annual Meeting of the Chemical Society Japan, Nagoya.
3/14/2014: We joined the Molecular Robotic meeting (Iwanuma).
3/13/2014: Dr. Ioanna Mela (Univ. Cambridge) visited our group and carried out the research.
2/3/2014: Prof. Jean-Jacques Toulmé (Univ. Bordeaux) visited our group and gave lecture.
1/9/2014: We joined the 2nd Kyoto-Bristol symposium (Kyoto).
11/13/2013: We joined the 40th International Symposium on Nucleic Acid Chemistry (ISNAC), Yokohama. Yuki Suzuki (Postdoctoral Fellow) won the Outstanding Poster Presentation Award,"Dynamic assembly/disassembly processes of photoresponsive DNA origami nanostructures captured by high-speed atomic force microscopy".
11/9/2013: Endo gave a talk in Japan Society for Bioscience, Biotechnology, and Agrochemistry, Chubu branch 169th annual symposium (Gifu)
11/6/2013: We joined the 7th Annual Symposium on Nanobiotechnology (Bristol, UK).
11/1/2013: Endo joined and talked in the 10th Japanese-German Frontiers of Science (JGFoS) Symposium (Kyoto).
10/29/2013: Prof. Peng Yin (Harvard Medical School) visited our group and gave lecture.
10/29/2013: We joined the 51st Annual Meeting of the Biophysical Society of Japan, Kyoto.
10/10/2013: We joined UK-Japan Workshop on Organic-Inorganic Framework Materilas, Kyoto.
9/27/2013: We joined the 7th Symposium on Biorelevant Chemistry, Nagoya.
9/26/2013: Ms. Marisa Götzfried (Technische Universität München) joined our group (interunversity exchange program) and conducted research. 　　　　　　　
8/20/2013: Dr. Laura Na Liu (Max Planck Institute) visited our group.
7/20/2013: We joined the reunion party of Sugiyama Lab.
6/24/2013: Yangyang Yang won The 3rd Takegoshi Prize from Graduate School of Science, Kyoto University.
6/21/2012: We joined the 8th Annual Meeting of Japanese Society for Chemical Biology, Tokyo.
6/10/2013: Yangyang Yang visited Baoquan Ding Lab. (National Center for Nano Science and Technology of China, Beijing).
6/1/2013: Dr. Rajendran moved to Komiyama Lab. Tsukuba Advanced Research Alliance, The University of Tsukuba, as a Project Assistant Professor.
4/15/2013: We joined FNANO13 (Snowbird, UT, USA).
3/22/2013: We joined the 93th Annual Meeting of the Chemical Society Japan, Shiga.
3/18/2013: We joined RSC Publishing-iCeMS Joint International Symposium, Kyoto.
2/22/2013: Endo gave a talk in a seminar at Department of Material Sciences, JAIST (Ishikawa).
2/7/2013: Seigi Yamamoto visited Hanbin Mao Lab. (Kent State University, Ohio).
11/28/2012: Prof. Itamar Willner (The Hebrew University of Jerusalem) visited our group and gave lecture.
11/17/2012: We joined the 39th International Symposium on Nucleic Acid Chemistry (ISNAC), Nagoya.
Yuki Suzuki (Postdoctoral Fellow) won the Outstanding Poster Presentation Award, "Direct visualization of Cre-mediated site-specific recombination in the DNA origami scaffold".
11/17/2012: We joined the 6th Annual Symposium on Nanobiotechnology Kyoto Cell Material Integration (Kyoto).
10/19/2012: Yangyang Yang (graduate student) got JSPS pre-doctoral fellowship.
10/1/2012: Mr. Deepak Koirala (Kent State University) visited for collaborative reserach.
9/6/2012: We joined the 6th Symposium on Biorelevant Chemistry, Sapporo.
8/13/2012: We joined dnatec2012 & dna18 (Aarhus, Denmark).
7/13/2012: Endo gave a talk in a seminar at Department of Pharmaceutical Sciences, Osaka University (Suita).
7/9/2012: ACS Chemical & Engineering News (Volume 90, Issue 28, July 9, 2012, pp.30-31),"More Than Just A Smiley Face".
6/9/2012: We joined the 7th Annual Meeting of Japanese Society for Chemical Biology, Kyoto.
4/20/2012: We joined CLS-iCeMS Joint Symposium (Peking University, Tsinghua University, Beijing).
4/18/2012: We joined FNANO12 (Snowbird, UT, USA).
3/28/2012: We joined the 92th Annual Meeting of the Chemical Society Japan, Yokohama. Yousuke Katsuda got the Outstanding Presentation, titled "To reveal the Cre recombinase reaction mechanism using a defined DNA nanostructure".
2/6/2012: ACS Chemical & Engineering News (Volume 90, Issue 6, February 6, 2012, pp.31), "DNA Nanostructure Controls Transcription".
1/13/2012: Kyoto University Press Release, tilttled "Regulation of the movement of DNA motor in nanoscale space".
11/17/2011: We joined the 5th Anual Symposium on Nanobiotechnology, Seoul NanoHealth 2011 (Yonsei University, Seoul, Korea).
11/16/2011: We joined POSTECH AMS Symposium on Nanotechnology (Pohang University of Science and Technology, Pohang, Korea).
11/9/2011: We joined the 38th International Symposium on Nucleic Acid Chemistry (ISNAC), Sapporo.
Arivazhagan Rajendran (Postdoctoral Fellow) won the Outstanding Poster Presentation Award, "Programmed Self-Assembly and Thermal-Stabilization of DNA Origami Nanostructures".
9/12/2011: We joined the 5th Symposium on Biorelevant Chemistry, Tsukuba.
5/16/2011: We joined the 6th Annual Meeting of Japanese Society for Chmical Biology, Tokyo.
4/18/2011: Yousuke Katsuda visited Barry Gold Lab. (University of Pittsburgh).
4/11/2011: We joined FNANO11 (Snowbird, UT, USA).
2/7/2011: Kyoto University Press Release, tilttled "Real-time observation of DNA motor for development of molecular robot in nano- and mesoscale".　　
12/10/2010: We joined the International Chemical Congress of Pacific Basin Societies, (Honolulu, Hawaii, USA)
11/12/2010: We joined the 37th International Symposium on Nucleic Acid Chemistry (ISNAC), Yokohama. Yuta Sannohe got the Outstanding Poster Presentation Award, "Dynamic conformational switching via G-quadruplex formation observed in the DNA nanostructure".
11/9/2010: We held the 8th iCeMS International Symposium and invited Prof. William Shih and Prof. Kurt Gothelf.
5/19/2010: We joined the 5th Annual Meeting of Japanese Society for Chmical Biology, Yokohama.
4/13/2010: We joined FNANO10 (Snowbird, UT, USA).
3/29/2010: We joined the 90th Annual Meeting of the Chemical Society Japan, Osaka.Yousuke Katsuda got the Outstanding Presentation, tittled "Regulation of DNA modifying and repair enzymes in a nanostructure".
2/13/2010: We held the 7th iCeMS Cafe "Weave DNA like an origami".
1/15/2010: Kyoto University Press Release, tilttled "Control of the eyzyme reactions and the direct observation in the DNA nano-space".
10/1/2009: We joined the 36th International Symposium on Nucleic Acid Chemistry(ISNAC), Takayama.